KMID : 0545120120220050600
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Journal of Microbiology and Biotechnology 2012 Volume.22 No. 5 p.600 ~ p.606
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Identification of Chinese Cabbage Sentrin as a Suppressor of Bax-Induced Cell Death in Yeast
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Widhi Dyah Sawitri
Slameto Slameto Bambang Sugiharto Kim Kyung-Min
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Abstract
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Studies into the cell death program termed apoptosis have resulted in new information regarding how cells control and execute their own demise, including insights into the mechanism by which death-preventing factors can inhibit Bax-induced caspase activation. We investigated high temperature stress-induced cell death in Brassica rapa. Using a yeast functional screening from a Brassica rapa cDNA library, the BH5-127 EST clone encoding an apoptotic suppressor peptide was identified. However, a phylogenic tree showed that BH5-127 clusters within a clade containing SUMO-1 (Small Ubiquitin-like Modifier- 1). BH5-127 was confirmed similar to have function to SUMO-1 as Fas suppression. Expression of BH5-127 showed that substantial suppression of cell death survived on SD-galactose-Leu--Ura- medium. The results suggest that BrSE (Brassica rapa Sentrin EST, BH5-127) is one of the important regulatory proteins in programming cell death, especially in the seedling stage of Chinese cabbage.
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KEYWORD
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apoptosis, Brassica rapa, sentrin, SUMO-1, yeast functional screening
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